Construct: shRNA TRCN0000073973

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006463.3-1929s1c1
DNA Barcode:
GCAATATGAATGGAGCTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAMBP (10617)
Gene Description:
STAM binding protein
Transcript:
RefSeq NM_006463.3 (NON-CURRENT)
Match location:
Position 1929 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000073973)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATATGAATGGAGCTTATT
Hairpin Sequence:
5'-CCGG-GCAATATGAATGGAGCTTATT-CTCGAG-AATAAGCTCCATTCATATTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATATGAATGGAGCTTATTCTCGAGAATAAGCTCCATTCATATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATATGAATGGAGCTTATTCTCGAGAATAAGCTCCATTCATATTGC-3'

Other clones with same target sequence:

  1. TRCN0000299500