Construct: shRNA TRCN0000074133

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198568.1-1927s1c1
DNA Barcode:
GCCCACTTGATAATTACTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GJB7 (375519)
Gene Description:
gap junction protein beta 7
Transcript:
RefSeq NM_198568.1 (NON-CURRENT)
Match location:
Position 1927 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074133)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCACTTGATAATTACTTAA
Hairpin Sequence:
5'-CCGG-GCCCACTTGATAATTACTTAA-CTCGAG-TTAAGTAATTATCAAGTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCACTTGATAATTACTTAACTCGAGTTAAGTAATTATCAAGTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCACTTGATAATTACTTAACTCGAGTTAAGTAATTATCAAGTGGGC-3'

Other clones with same target sequence:

(none)