Construct: shRNA TRCN0000074181

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003687.2-941s1c1
DNA Barcode:
CTACTGTGAGAGCCACGCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDLIM4 (8572)
Gene Description:
PDZ and LIM domain 4
Transcript:
RefSeq NM_003687.2 (NON-CURRENT)
Match location:
Position 941 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074181)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACTGTGAGAGCCACGCCAA
Hairpin Sequence:
5'-CCGG-CTACTGTGAGAGCCACGCCAA-CTCGAG-TTGGCGTGGCTCTCACAGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACTGTGAGAGCCACGCCAACTCGAGTTGGCGTGGCTCTCACAGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACTGTGAGAGCCACGCCAACTCGAGTTGGCGTGGCTCTCACAGTAG-3'

Other clones with same target sequence:

(none)