Construct: shRNA TRCN0000074192

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001321.1-587s1c1
DNA Barcode:
AGGATGCTATGCAAAGAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSRP2 (1466)
Gene Description:
cysteine and glycine rich protein 2
Transcript:
RefSeq NM_001321.1 (NON-CURRENT)
Match location:
Position 587 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074192)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGATGCTATGCAAAGAACTT
Hairpin Sequence:
5'-CCGG-AGGATGCTATGCAAAGAACTT-CTCGAG-AAGTTCTTTGCATAGCATCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGATGCTATGCAAAGAACTTCTCGAGAAGTTCTTTGCATAGCATCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGATGCTATGCAAAGAACTTCTCGAGAAGTTCTTTGCATAGCATCCT-3'

Other clones with same target sequence:

(none)