Construct: shRNA TRCN0000074249

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175065.2-250s1c1
DNA Barcode:
CTGCAACTAGCCGTGAGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H2AC21 (317772)
Gene Description:
H2A clustered histone 21
Transcript:
RefSeq NM_175065.2 (CURRENT)
Match location:
Position 250 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074249)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCAACTAGCCGTGAGGAAT
Hairpin Sequence:
5'-CCGG-CTGCAACTAGCCGTGAGGAAT-CTCGAG-ATTCCTCACGGCTAGTTGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCAACTAGCCGTGAGGAATCTCGAGATTCCTCACGGCTAGTTGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCAACTAGCCGTGAGGAATCTCGAGATTCCTCACGGCTAGTTGCAG-3'

Other clones with same target sequence:

(none)