Construct: shRNA TRCN0000074333

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018061.1-1925s1c1
DNA Barcode:
GCACAGATTGTTCTTGCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRPF38B (55119)
Gene Description:
pre-mRNA processing factor 38B
Transcript:
RefSeq NM_018061.1 (NON-CURRENT)
Match location:
Position 1925 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074333)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGATTGTTCTTGCATTT
Hairpin Sequence:
5'-CCGG-GCACAGATTGTTCTTGCATTT-CTCGAG-AAATGCAAGAACAATCTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGATTGTTCTTGCATTTCTCGAGAAATGCAAGAACAATCTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGATTGTTCTTGCATTTCTCGAGAAATGCAAGAACAATCTGTGC-3'

Other clones with same target sequence:

(none)