Construct: shRNA TRCN0000074334

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018061.1-500s1c1
DNA Barcode:
CGAAAGCAAGTGATGGGTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRPF38B (55119)
Gene Description:
pre-mRNA processing factor 38B
Transcript:
RefSeq NM_018061.1 (NON-CURRENT)
Match location:
Position 500 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074334)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAAGCAAGTGATGGGTCTT
Hairpin Sequence:
5'-CCGG-CGAAAGCAAGTGATGGGTCTT-CTCGAG-AAGACCCATCACTTGCTTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAAGCAAGTGATGGGTCTTCTCGAGAAGACCCATCACTTGCTTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAAGCAAGTGATGGGTCTTCTCGAGAAGACCCATCACTTGCTTTCG-3'

Other clones with same target sequence:

(none)