Construct: shRNA TRCN0000074358

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178014.2-1617s1c1
DNA Barcode:
GCGCTCAATAAATACTTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TUBB (203068)
Gene Description:
tubulin beta class I
Transcript:
RefSeq NM_178014.2 (NON-CURRENT)
Match location:
Position 1617 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074358)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCTCAATAAATACTTGTTT
Hairpin Sequence:
5'-CCGG-GCGCTCAATAAATACTTGTTT-CTCGAG-AAACAAGTATTTATTGAGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCTCAATAAATACTTGTTTCTCGAGAAACAAGTATTTATTGAGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCTCAATAAATACTTGTTTCTCGAGAAACAAGTATTTATTGAGCGC-3'

Other clones with same target sequence:

  1. TRCN0000311723