Construct: shRNA TRCN0000074383

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004640.4-883s1c1
DNA Barcode:
CCTCAACCTCAAACACATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDX39B (7919)
Gene Description:
DExD-box helicase 39B
Transcript:
RefSeq NM_004640.4 (NON-CURRENT)
Match location:
Position 883 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074383)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAACCTCAAACACATTAA
Hairpin Sequence:
5'-CCGG-CCTCAACCTCAAACACATTAA-CTCGAG-TTAATGTGTTTGAGGTTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAACCTCAAACACATTAACTCGAGTTAATGTGTTTGAGGTTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAACCTCAAACACATTAACTCGAGTTAATGTGTTTGAGGTTGAGG-3'

Other clones with same target sequence:

  1. TRCN0000286976