Construct: shRNA TRCN0000074400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004597.4-425s1c1
DNA Barcode:
CATCAACTGCCGCAACAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNRPD2 (6633)
Gene Description:
small nuclear ribonucleoprotein D2 polypeptide
Transcript:
RefSeq NM_004597.4 (NON-CURRENT)
Match location:
Position 425 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCAACTGCCGCAACAATAA
Hairpin Sequence:
5'-CCGG-CATCAACTGCCGCAACAATAA-CTCGAG-TTATTGTTGCGGCAGTTGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCAACTGCCGCAACAATAACTCGAGTTATTGTTGCGGCAGTTGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCAACTGCCGCAACAATAACTCGAGTTATTGTTGCGGCAGTTGATG-3'

Other clones with same target sequence:

  1. TRCN0000301102