Construct: shRNA TRCN0000074431

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021938.2-320s1c1
DNA Barcode:
GCCAGGGATTCCGCCATCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CELF5 (60680)
Gene Description:
CUGBP Elav-like family member 5
Transcript:
RefSeq NM_021938.2 (NON-CURRENT)
Match location:
Position 320 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074431)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGGGATTCCGCCATCAAA
Hairpin Sequence:
5'-CCGG-GCCAGGGATTCCGCCATCAAA-CTCGAG-TTTGATGGCGGAATCCCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGGGATTCCGCCATCAAACTCGAGTTTGATGGCGGAATCCCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGGGATTCCGCCATCAAACTCGAGTTTGATGGCGGAATCCCTGGC-3'

Other clones with same target sequence:

(none)