Construct: shRNA TRCN0000074435

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016652.3-830s1c1
DNA Barcode:
GCGTGCTTTAGATGTAGACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRNKL1 (51340)
Gene Description:
crooked neck pre-mRNA splicing factor 1
Transcript:
RefSeq NM_016652.3 (NON-CURRENT)
Match location:
Position 830 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074435)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTGCTTTAGATGTAGACTA
Hairpin Sequence:
5'-CCGG-GCGTGCTTTAGATGTAGACTA-CTCGAG-TAGTCTACATCTAAAGCACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTGCTTTAGATGTAGACTACTCGAGTAGTCTACATCTAAAGCACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTGCTTTAGATGTAGACTACTCGAGTAGTCTACATCTAAAGCACGC-3'

Other clones with same target sequence:

(none)