Construct: shRNA TRCN0000074440

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173491.1-571s1c1
DNA Barcode:
GCATTCGACAAGTTCTGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LSM11 (134353)
Gene Description:
LSM11, U7 small nuclear RNA associated
Transcript:
RefSeq NM_173491.1 (NON-CURRENT)
Match location:
Position 571 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074440)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTCGACAAGTTCTGGAAT
Hairpin Sequence:
5'-CCGG-GCATTCGACAAGTTCTGGAAT-CTCGAG-ATTCCAGAACTTGTCGAATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTCGACAAGTTCTGGAATCTCGAGATTCCAGAACTTGTCGAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTCGACAAGTTCTGGAATCTCGAGATTCCAGAACTTGTCGAATGC-3'

Other clones with same target sequence:

(none)