Construct: shRNA TRCN0000074490
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_024704.3-3628s1c1
- DNA Barcode:
- GCACCATTCAACGTAAACTAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KIF16B (55614)
- Gene Description:
- kinesin family member 16B
- Transcript:
- RefSeq NM_024704.3 (NON-CURRENT)
- Match location:
- Position 3628 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000074490)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCACCATTCAACGTAAACTAA
- Hairpin Sequence:
-
5'-CCGG-GCACCATTCAACGTAAACTAA-CTCGAG-TTAGTTTACGTTGAATGGTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCACCATTCAACGTAAACTAACTCGAGTTAGTTTACGTTGAATGGTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCACCATTCAACGTAAACTAACTCGAGTTAGTTTACGTTGAATGGTGC-3'
Other clones with same target sequence:
-
TRCN0000289596
