Construct: shRNA TRCN0000074548

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013293.2-1245s1c1
DNA Barcode:
CCCAATGTTATGTTATGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRA2A (29896)
Gene Description:
transformer 2 alpha homolog
Transcript:
RefSeq NM_013293.2 (NON-CURRENT)
Match location:
Position 1245 (3UTR)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074548)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATGTTATGTTATGCTTT
Hairpin Sequence:
5'-CCGG-CCCAATGTTATGTTATGCTTT-CTCGAG-AAAGCATAACATAACATTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATGTTATGTTATGCTTTCTCGAGAAAGCATAACATAACATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATGTTATGTTATGCTTTCTCGAGAAAGCATAACATAACATTGGG-3'

Other clones with same target sequence:

(none)