Construct: shRNA TRCN0000074573

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003512.3-126s1c1
DNA Barcode:
CCAAAGCGAAATCCCGCTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H2AC6 (8334)
Gene Description:
H2A clustered histone 6
Transcript:
RefSeq NM_003512.3 (CURRENT)
Match location:
Position 126 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074573)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGCGAAATCCCGCTCTT
Hairpin Sequence:
5'-CCGG-CCAAAGCGAAATCCCGCTCTT-CTCGAG-AAGAGCGGGATTTCGCTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGCGAAATCCCGCTCTTCTCGAGAAGAGCGGGATTTCGCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGCGAAATCCCGCTCTTCTCGAGAAGAGCGGGATTTCGCTTTGG-3'

Other clones with same target sequence:

(none)