Construct: shRNA TRCN0000074578

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005212.1-661s1c1
DNA Barcode:
GCTGAAACCAAATTACTACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSN3 (1448)
Gene Description:
casein kappa
Transcript:
RefSeq NM_005212.1 (NON-CURRENT)
Match location:
Position 661 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074578)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAAACCAAATTACTACTT
Hairpin Sequence:
5'-CCGG-GCTGAAACCAAATTACTACTT-CTCGAG-AAGTAGTAATTTGGTTTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAAACCAAATTACTACTTCTCGAGAAGTAGTAATTTGGTTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAAACCAAATTACTACTTCTCGAGAAGTAGTAATTTGGTTTCAGC-3'

Other clones with same target sequence:

(none)