Construct: shRNA TRCN0000074639

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002568.3-2019s1c1
DNA Barcode:
CCGCACCGTTCCACAGTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PABPC1 (26986)
Gene Description:
poly(A) binding protein cytoplasmic 1
Transcript:
RefSeq NM_002568.3 (NON-CURRENT)
Match location:
Position 2019 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074639)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCACCGTTCCACAGTATAA
Hairpin Sequence:
5'-CCGG-CCGCACCGTTCCACAGTATAA-CTCGAG-TTATACTGTGGAACGGTGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCACCGTTCCACAGTATAACTCGAGTTATACTGTGGAACGGTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCACCGTTCCACAGTATAACTCGAGTTATACTGTGGAACGGTGCGG-3'

Other clones with same target sequence:

  1. TRCN0000298167