Construct: shRNA TRCN0000074673
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_006547.2-3584s1c1
- DNA Barcode:
- GCCTCATTCTTATTTCAAGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IGF2BP3 (10643)
- Gene Description:
- insulin like growth factor 2 mRNA binding protein 3
- Transcript:
- RefSeq NM_006547.2 (NON-CURRENT)
- Match location:
- Position 3584 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCTCATTCTTATTTCAAGAT
- Hairpin Sequence:
-
5'-CCGG-GCCTCATTCTTATTTCAAGAT-CTCGAG-ATCTTGAAATAAGAATGAGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTCATTCTTATTTCAAGATCTCGAGATCTTGAAATAAGAATGAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTCATTCTTATTTCAAGATCTCGAGATCTTGAAATAAGAATGAGGC-3'
Other clones with same target sequence:
(none)
