Construct: shRNA TRCN0000074684

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005016.3-530s1c1
DNA Barcode:
CCCACTAATGCCATCTTCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCBP2 (5094)
Gene Description:
poly(rC) binding protein 2
Transcript:
RefSeq NM_005016.3 (NON-CURRENT)
Match location:
Position 530 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074684)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACTAATGCCATCTTCAAA
Hairpin Sequence:
5'-CCGG-CCCACTAATGCCATCTTCAAA-CTCGAG-TTTGAAGATGGCATTAGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACTAATGCCATCTTCAAACTCGAGTTTGAAGATGGCATTAGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACTAATGCCATCTTCAAACTCGAGTTTGAAGATGGCATTAGTGGG-3'

Other clones with same target sequence:

  1. TRCN0000327832