Construct: shRNA TRCN0000074692

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014977.1-3392s1c1
DNA Barcode:
CTTTCACTTTAGGCCAGCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACIN1 (22985)
Gene Description:
apoptotic chromatin condensation inducer 1
Transcript:
RefSeq NM_014977.1 (NON-CURRENT)
Match location:
Position 3392 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074692)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTCACTTTAGGCCAGCTAA
Hairpin Sequence:
5'-CCGG-CTTTCACTTTAGGCCAGCTAA-CTCGAG-TTAGCTGGCCTAAAGTGAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTCACTTTAGGCCAGCTAACTCGAGTTAGCTGGCCTAAAGTGAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTCACTTTAGGCCAGCTAACTCGAGTTAGCTGGCCTAAAGTGAAAG-3'

Other clones with same target sequence:

(none)