Construct: shRNA TRCN0000074698

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020528.1-1325s1c1
DNA Barcode:
CCTGCCTCACAGATACCAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCBP3 (54039)
Gene Description:
poly(rC) binding protein 3
Transcript:
RefSeq NM_020528.1 (NON-CURRENT)
Match location:
Position 1325 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074698)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCCTCACAGATACCAATA
Hairpin Sequence:
5'-CCGG-CCTGCCTCACAGATACCAATA-CTCGAG-TATTGGTATCTGTGAGGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCCTCACAGATACCAATACTCGAGTATTGGTATCTGTGAGGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCCTCACAGATACCAATACTCGAGTATTGGTATCTGTGAGGCAGG-3'

Other clones with same target sequence:

(none)