Construct: shRNA TRCN0000074707

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022768.4-2127s1c1
DNA Barcode:
GATCGTTACAACAGCGACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBM15 (64783)
Gene Description:
RNA binding motif protein 15
Transcript:
RefSeq NM_022768.4 (CURRENT)
Match location:
Position 2127 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074707)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCGTTACAACAGCGACAAT
Hairpin Sequence:
5'-CCGG-GATCGTTACAACAGCGACAAT-CTCGAG-ATTGTCGCTGTTGTAACGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCGTTACAACAGCGACAATCTCGAGATTGTCGCTGTTGTAACGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCGTTACAACAGCGACAATCTCGAGATTGTCGCTGTTGTAACGATC-3'

Other clones with same target sequence:

(none)