Construct: shRNA TRCN0000074715

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001007.3-718s1c1
DNA Barcode:
GTTCACGTGAAAGATGCCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS4X (6191)
Gene Description:
ribosomal protein S4 X-linked
Transcript:
RefSeq NM_001007.3 (NON-CURRENT)
Match location:
Position 718 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074715)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCACGTGAAAGATGCCAAT
Hairpin Sequence:
5'-CCGG-GTTCACGTGAAAGATGCCAAT-CTCGAG-ATTGGCATCTTTCACGTGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCACGTGAAAGATGCCAATCTCGAGATTGGCATCTTTCACGTGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCACGTGAAAGATGCCAATCTCGAGATTGGCATCTTTCACGTGAAC-3'

Other clones with same target sequence:

  1. TRCN0000289114