Construct: shRNA TRCN0000074719

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007080.1-276s1c1
DNA Barcode:
CCGAGGAAACAATGTGTTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LSM6 (11157)
Gene Description:
LSM6 homolog, U6 small nuclear RNA and mRNA degradation associated
Transcript:
RefSeq NM_007080.1 (NON-CURRENT)
Match location:
Position 276 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074719)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGGAAACAATGTGTTGTA
Hairpin Sequence:
5'-CCGG-CCGAGGAAACAATGTGTTGTA-CTCGAG-TACAACACATTGTTTCCTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGGAAACAATGTGTTGTACTCGAGTACAACACATTGTTTCCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGGAAACAATGTGTTGTACTCGAGTACAACACATTGTTTCCTCGG-3'

Other clones with same target sequence:

(none)