Construct: shRNA TRCN0000074721

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007080.1-129s1c1
DNA Barcode:
CGGACGACCAGTTGTGGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LSM6 (11157)
Gene Description:
LSM6 homolog, U6 small nuclear RNA and mRNA degradation associated
Transcript:
RefSeq NM_007080.1 (NON-CURRENT)
Match location:
Position 129 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074721)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACGACCAGTTGTGGTAAA
Hairpin Sequence:
5'-CCGG-CGGACGACCAGTTGTGGTAAA-CTCGAG-TTTACCACAACTGGTCGTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACGACCAGTTGTGGTAAACTCGAGTTTACCACAACTGGTCGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACGACCAGTTGTGGTAAACTCGAGTTTACCACAACTGGTCGTCCG-3'

Other clones with same target sequence:

(none)