Construct: shRNA TRCN0000074740

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001008.2-154s1c1
DNA Barcode:
CTCAGGAATAGACTCAAGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS4Y1 (6192)
Gene Description:
ribosomal protein S4 Y-linked 1
Transcript:
RefSeq NM_001008.2 (NON-CURRENT)
Match location:
Position 154 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074740)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAGGAATAGACTCAAGTAT
Hairpin Sequence:
5'-CCGG-CTCAGGAATAGACTCAAGTAT-CTCGAG-ATACTTGAGTCTATTCCTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGGAATAGACTCAAGTATCTCGAGATACTTGAGTCTATTCCTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGGAATAGACTCAAGTATCTCGAGATACTTGAGTCTATTCCTGAG-3'

Other clones with same target sequence:

(none)