Construct: shRNA TRCN0000074750

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021239.1-1538s1c1
DNA Barcode:
GCGCCTTAAGAATTGGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBM25 (58517)
Gene Description:
RNA binding motif protein 25
Transcript:
RefSeq NM_021239.1 (NON-CURRENT)
Match location:
Position 1538 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074750)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCCTTAAGAATTGGGAAAT
Hairpin Sequence:
5'-CCGG-GCGCCTTAAGAATTGGGAAAT-CTCGAG-ATTTCCCAATTCTTAAGGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCCTTAAGAATTGGGAAATCTCGAGATTTCCCAATTCTTAAGGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCCTTAAGAATTGGGAAATCTCGAGATTTCCCAATTCTTAAGGCGC-3'

Other clones with same target sequence:

  1. TRCN0000286633