Construct: shRNA TRCN0000074771

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004697.3-1238s1c1
DNA Barcode:
AGGACGTTGTATCATGTTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRPF4 (9128)
Gene Description:
pre-mRNA processing factor 4
Transcript:
RefSeq NM_004697.3 (NON-CURRENT)
Match location:
Position 1238 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074771)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGACGTTGTATCATGTTCTT
Hairpin Sequence:
5'-CCGG-AGGACGTTGTATCATGTTCTT-CTCGAG-AAGAACATGATACAACGTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGACGTTGTATCATGTTCTTCTCGAGAAGAACATGATACAACGTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGACGTTGTATCATGTTCTTCTCGAGAAGAACATGATACAACGTCCT-3'

Other clones with same target sequence:

(none)