Construct: shRNA TRCN0000074810

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001014.2-233s1c1
DNA Barcode:
CTGGAGACATTTCTACTGGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS10 (6204)
Gene Description:
ribosomal protein S10
Transcript:
RefSeq NM_001014.2 (NON-CURRENT)
Match location:
Position 233 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074810)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGAGACATTTCTACTGGTA
Hairpin Sequence:
5'-CCGG-CTGGAGACATTTCTACTGGTA-CTCGAG-TACCAGTAGAAATGTCTCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGAGACATTTCTACTGGTACTCGAGTACCAGTAGAAATGTCTCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGAGACATTTCTACTGGTACTCGAGTACCAGTAGAAATGTCTCCAG-3'

Other clones with same target sequence:

  1. TRCN0000298184