Construct: shRNA TRCN0000074822

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021144.2-182s1c1
DNA Barcode:
ACCCACAAACAAACTACCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSIP1 (11168)
Gene Description:
PC4 and SFRS1 interacting protein 1
Transcript:
RefSeq NM_021144.2 (NON-CURRENT)
Match location:
Position 182 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074822)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCACAAACAAACTACCCAT
Hairpin Sequence:
5'-CCGG-ACCCACAAACAAACTACCCAT-CTCGAG-ATGGGTAGTTTGTTTGTGGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCACAAACAAACTACCCATCTCGAGATGGGTAGTTTGTTTGTGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCACAAACAAACTACCCATCTCGAGATGGGTAGTTTGTTTGTGGGT-3'

Other clones with same target sequence:

(none)