Construct: shRNA TRCN0000074846

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016091.2-786s1c1
DNA Barcode:
CCTGGTAGACAAATCCAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EIF3L (51386)
Gene Description:
eukaryotic translation initiation factor 3 subunit L
Transcript:
RefSeq NM_016091.2 (NON-CURRENT)
Match location:
Position 786 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074846)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGTAGACAAATCCAACAT
Hairpin Sequence:
5'-CCGG-CCTGGTAGACAAATCCAACAT-CTCGAG-ATGTTGGATTTGTCTACCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGTAGACAAATCCAACATCTCGAGATGTTGGATTTGTCTACCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGTAGACAAATCCAACATCTCGAGATGTTGGATTTGTCTACCAGG-3'

Other clones with same target sequence:

  1. TRCN0000307937