Construct: shRNA TRCN0000074888

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006047.4-4025s1c1
DNA Barcode:
GCCCATTCATAAGAAAGTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBM12 (10137)
Gene Description:
RNA binding motif protein 12
Transcript:
RefSeq NM_006047.4 (NON-CURRENT)
Match location:
Position 4025 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074888)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATTCATAAGAAAGTGTT
Hairpin Sequence:
5'-CCGG-GCCCATTCATAAGAAAGTGTT-CTCGAG-AACACTTTCTTATGAATGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATTCATAAGAAAGTGTTCTCGAGAACACTTTCTTATGAATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATTCATAAGAAAGTGTTCTCGAGAACACTTTCTTATGAATGGGC-3'

Other clones with same target sequence:

(none)