Construct: shRNA TRCN0000074912

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032284.2-427s1c1
DNA Barcode:
CCGGAGAAGCTACCGAGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRPF38A (84950)
Gene Description:
pre-mRNA processing factor 38A
Transcript:
RefSeq NM_032284.2 (NON-CURRENT)
Match location:
Position 427 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074912)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAGAAGCTACCGAGACTT
Hairpin Sequence:
5'-CCGG-CCGGAGAAGCTACCGAGACTT-CTCGAG-AAGTCTCGGTAGCTTCTCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAGAAGCTACCGAGACTTCTCGAGAAGTCTCGGTAGCTTCTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAGAAGCTACCGAGACTTCTCGAGAAGTCTCGGTAGCTTCTCCGG-3'

Other clones with same target sequence:

(none)