Construct: shRNA TRCN0000074921

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014612.2-776s1c1
DNA Barcode:
GCTTCCTTTCACTGGAGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM120A (23196)
Gene Description:
family with sequence similarity 120A
Transcript:
RefSeq NM_014612.2 (NON-CURRENT)
Match location:
Position 776 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074921)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCCTTTCACTGGAGTTTA
Hairpin Sequence:
5'-CCGG-GCTTCCTTTCACTGGAGTTTA-CTCGAG-TAAACTCCAGTGAAAGGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCCTTTCACTGGAGTTTACTCGAGTAAACTCCAGTGAAAGGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCCTTTCACTGGAGTTTACTCGAGTAAACTCCAGTGAAAGGAAGC-3'

Other clones with same target sequence:

(none)