Construct: shRNA TRCN0000074938

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032141.1-2036s1c1
DNA Barcode:
CGTGCCTGTAATACCAACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NSRP1 (84081)
Gene Description:
nuclear speckle splicing regulatory protein 1
Transcript:
RefSeq NM_032141.1 (NON-CURRENT)
Match location:
Position 2036 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074938)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGCCTGTAATACCAACATT
Hairpin Sequence:
5'-CCGG-CGTGCCTGTAATACCAACATT-CTCGAG-AATGTTGGTATTACAGGCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGCCTGTAATACCAACATTCTCGAGAATGTTGGTATTACAGGCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGCCTGTAATACCAACATTCTCGAGAATGTTGGTATTACAGGCACG-3'

Other clones with same target sequence:

(none)