Construct: shRNA TRCN0000074950

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005872.2-218s1c1
DNA Barcode:
CCCGGATTATTCTGCCTTTGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BCAS2 (10286)
Gene Description:
BCAS2 pre-mRNA processing factor
Transcript:
RefSeq NM_005872.2 (NON-CURRENT)
Match location:
Position 218 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074950)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGGATTATTCTGCCTTTGA
Hairpin Sequence:
5'-CCGG-CCCGGATTATTCTGCCTTTGA-CTCGAG-TCAAAGGCAGAATAATCCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGGATTATTCTGCCTTTGACTCGAGTCAAAGGCAGAATAATCCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGGATTATTCTGCCTTTGACTCGAGTCAAAGGCAGAATAATCCGGG-3'

Other clones with same target sequence:

  1. TRCN0000290972