Construct: shRNA TRCN0000074974

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144716.1-194s1c1
DNA Barcode:
CGGAACTATGTCCCGGAGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC12 (151903)
Gene Description:
coiled-coil domain containing 12
Transcript:
RefSeq NM_144716.1 (NON-CURRENT)
Match location:
Position 194 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074974)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAACTATGTCCCGGAGGAT
Hairpin Sequence:
5'-CCGG-CGGAACTATGTCCCGGAGGAT-CTCGAG-ATCCTCCGGGACATAGTTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAACTATGTCCCGGAGGATCTCGAGATCCTCCGGGACATAGTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAACTATGTCCCGGAGGATCTCGAGATCCTCCGGGACATAGTTCCG-3'

Other clones with same target sequence:

(none)