Construct: shRNA TRCN0000074993

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000969.2-212s1c1
DNA Barcode:
GATGATAGTTCGTGTGACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPL5 (6125)
Gene Description:
ribosomal protein L5
Transcript:
RefSeq NM_000969.2 (NON-CURRENT)
Match location:
Position 212 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074993)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGATAGTTCGTGTGACAAA
Hairpin Sequence:
5'-CCGG-GATGATAGTTCGTGTGACAAA-CTCGAG-TTTGTCACACGAACTATCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGATAGTTCGTGTGACAAACTCGAGTTTGTCACACGAACTATCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGATAGTTCGTGTGACAAACTCGAGTTTGTCACACGAACTATCATC-3'

Other clones with same target sequence:

  1. TRCN0000291974