Construct: shRNA TRCN0000074997

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000969.2-581s1c1
DNA Barcode:
CCCTCACAGTACCAAACGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPL5 (6125)
Gene Description:
ribosomal protein L5
Transcript:
RefSeq NM_000969.2 (NON-CURRENT)
Match location:
Position 581 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074997)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCACAGTACCAAACGATT
Hairpin Sequence:
5'-CCGG-CCCTCACAGTACCAAACGATT-CTCGAG-AATCGTTTGGTACTGTGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCACAGTACCAAACGATTCTCGAGAATCGTTTGGTACTGTGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCACAGTACCAAACGATTCTCGAGAATCGTTTGGTACTGTGAGGG-3'

Other clones with same target sequence:

  1. TRCN0000291973