Construct: shRNA TRCN0000075031

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000984.4-167s1c1
DNA Barcode:
CCTCCTAAAGCTGAAGCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPL23A (6147)
Gene Description:
ribosomal protein L23a
Transcript:
RefSeq NM_000984.4 (NON-CURRENT)
Match location:
Position 167 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075031)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCTAAAGCTGAAGCCAAA
Hairpin Sequence:
5'-CCGG-CCTCCTAAAGCTGAAGCCAAA-CTCGAG-TTTGGCTTCAGCTTTAGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCTAAAGCTGAAGCCAAACTCGAGTTTGGCTTCAGCTTTAGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCTAAAGCTGAAGCCAAACTCGAGTTTGGCTTCAGCTTTAGGAGG-3'

Other clones with same target sequence:

(none)