Construct: shRNA TRCN0000075041

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000993.2-213s1c1
DNA Barcode:
GCGCATTGACACCAGGCTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPL31 (6160)
Gene Description:
ribosomal protein L31
Transcript:
RefSeq NM_000993.2 (NON-CURRENT)
Match location:
Position 213 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075041)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCATTGACACCAGGCTCAA
Hairpin Sequence:
5'-CCGG-GCGCATTGACACCAGGCTCAA-CTCGAG-TTGAGCCTGGTGTCAATGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCATTGACACCAGGCTCAACTCGAGTTGAGCCTGGTGTCAATGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCATTGACACCAGGCTCAACTCGAGTTGAGCCTGGTGTCAATGCGC-3'

Other clones with same target sequence:

  1. TRCN0000307902