Construct: shRNA TRCN0000075044

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002092.2-416s1c1
DNA Barcode:
CGGTGAGAATGGAATACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRSF1 (2926)
Gene Description:
G-rich RNA sequence binding factor 1
Transcript:
RefSeq NM_002092.2 (NON-CURRENT)
Match location:
Position 416 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075044)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGAGAATGGAATACATTT
Hairpin Sequence:
5'-CCGG-CGGTGAGAATGGAATACATTT-CTCGAG-AAATGTATTCCATTCTCACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGAGAATGGAATACATTTCTCGAGAAATGTATTCCATTCTCACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGAGAATGGAATACATTTCTCGAGAAATGTATTCCATTCTCACCG-3'

Other clones with same target sequence:

  1. TRCN0000286172