Construct: shRNA TRCN0000075112

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006445.2-3459s1c1
DNA Barcode:
CGCCTCATGAAACATGATGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRPF8 (10594)
Gene Description:
pre-mRNA processing factor 8
Transcript:
RefSeq NM_006445.2 (NON-CURRENT)
Match location:
Position 3459 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075112)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCTCATGAAACATGATGTT
Hairpin Sequence:
5'-CCGG-CGCCTCATGAAACATGATGTT-CTCGAG-AACATCATGTTTCATGAGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCTCATGAAACATGATGTTCTCGAGAACATCATGTTTCATGAGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCTCATGAAACATGATGTTCTCGAGAACATCATGTTTCATGAGGCG-3'

Other clones with same target sequence:

  1. TRCN0000290331