Construct: shRNA TRCN0000075116

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006107.2-1019s1c1
DNA Barcode:
GCCGAACATCAGACAGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LUC7L3 (51747)
Gene Description:
LUC7 like 3 pre-mRNA splicing factor
Transcript:
RefSeq NM_006107.2 (NON-CURRENT)
Match location:
Position 1019 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075116)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAACATCAGACAGAAGAT
Hairpin Sequence:
5'-CCGG-GCCGAACATCAGACAGAAGAT-CTCGAG-ATCTTCTGTCTGATGTTCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAACATCAGACAGAAGATCTCGAGATCTTCTGTCTGATGTTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAACATCAGACAGAAGATCTCGAGATCTTCTGTCTGATGTTCGGC-3'

Other clones with same target sequence:

  1. TRCN0000286216