Construct: shRNA TRCN0000075128

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020701.1-1364s1c1
DNA Barcode:
GAAGAATCAATTAGTGGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ISY1 (57461)
Gene Description:
ISY1 splicing factor homolog
Transcript:
RefSeq NM_020701.1 (NON-CURRENT)
Match location:
Position 1364 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075128)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAATCAATTAGTGGCAAA
Hairpin Sequence:
5'-CCGG-GAAGAATCAATTAGTGGCAAA-CTCGAG-TTTGCCACTAATTGATTCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAATCAATTAGTGGCAAACTCGAGTTTGCCACTAATTGATTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAATCAATTAGTGGCAAACTCGAGTTTGCCACTAATTGATTCTTC-3'

Other clones with same target sequence:

(none)