Construct: shRNA TRCN0000075139

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014306.2-468s1c1
DNA Barcode:
CCGCTTGCTAAGAACCAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RTCB (51493)
Gene Description:
RNA 2',3'-cyclic phosphate and 5'-OH ligase
Transcript:
RefSeq NM_014306.2 (NON-CURRENT)
Match location:
Position 468 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075139)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCTTGCTAAGAACCAATTT
Hairpin Sequence:
5'-CCGG-CCGCTTGCTAAGAACCAATTT-CTCGAG-AAATTGGTTCTTAGCAAGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCTTGCTAAGAACCAATTTCTCGAGAAATTGGTTCTTAGCAAGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCTTGCTAAGAACCAATTTCTCGAGAAATTGGTTCTTAGCAAGCGG-3'

Other clones with same target sequence:

  1. TRCN0000290259