Construct: shRNA TRCN0000075149

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006546.3-692s1c1
DNA Barcode:
GCAGTGGTGAATGTCACCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IGF2BP1 (10642)
Gene Description:
insulin like growth factor 2 mRNA binding protein 1
Transcript:
RefSeq NM_006546.3 (NON-CURRENT)
Match location:
Position 692 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075149)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGGTGAATGTCACCTAT
Hairpin Sequence:
5'-CCGG-GCAGTGGTGAATGTCACCTAT-CTCGAG-ATAGGTGACATTCACCACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGGTGAATGTCACCTATCTCGAGATAGGTGACATTCACCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGGTGAATGTCACCTATCTCGAGATAGGTGACATTCACCACTGC-3'

Other clones with same target sequence:

(none)