Construct: shRNA TRCN0000075203

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013247.3-2121s1c1
DNA Barcode:
GAAGAATCACAGAAACACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HTRA2 (27429)
Gene Description:
HtrA serine peptidase 2
Transcript:
RefSeq NM_013247.3 (NON-CURRENT)
Match location:
Position 2121 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075203)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAATCACAGAAACACTTT
Hairpin Sequence:
5'-CCGG-GAAGAATCACAGAAACACTTT-CTCGAG-AAAGTGTTTCTGTGATTCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAATCACAGAAACACTTTCTCGAGAAAGTGTTTCTGTGATTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAATCACAGAAACACTTTCTCGAGAAAGTGTTTCTGTGATTCTTC-3'

Other clones with same target sequence:

(none)