Construct: shRNA TRCN0000075218

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002056.1-2871s1c1
DNA Barcode:
GCTGCTTTGGACAACTGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GFPT1 (2673)
Gene Description:
glutamine--fructose-6-phosphate transaminase 1
Transcript:
RefSeq NM_002056.1 (NON-CURRENT)
Match location:
Position 2871 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075218)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTTTGGACAACTGACAA
Hairpin Sequence:
5'-CCGG-GCTGCTTTGGACAACTGACAA-CTCGAG-TTGTCAGTTGTCCAAAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTTTGGACAACTGACAACTCGAGTTGTCAGTTGTCCAAAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTTTGGACAACTGACAACTCGAGTTGTCAGTTGTCCAAAGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000363668